Journal: Scientific Reports

Article Title: Simultaneous Monitoring of Cell-surface Receptor and Tumor-targeted Photodynamic Therapy via TdT-initiated Poly-G-Quadruplexes

doi: 10.1038/s41598-018-23902-5

Figure Lengend Snippet: Confocal microscopy images of incubated with poly-G-quadruplexes-ThT probe treated A549 ( a ) and HepG2 ( b ). Scale bar: 20 µm. ( c ) The normalized fluorescence intensity of individual cells was quantified from ( a ) and ( b ). Error bars indicate SD, n = 5. **P < 0.01. ( d ) Flow cytometry analysis of Cy5 labeled aptamer-primer with A549 and HepG2 cells, respectively.

Article Snippet: Live cell imaging was performed under confocal laser scanning microscope (CLSM) (Nikon, Eclipse TE2000-E) with a 60× oil immersion objective (Olympus, Melville, NY).

Techniques: Confocal Microscopy, Incubation, Fluorescence, Flow Cytometry, Labeling

Journal: PLoS ONE

Article Title: The STF2p Hydrophilin from Saccharomyces cerevisiae Is Required for Dehydration Stress Tolerance

doi: 10.1371/journal.pone.0033324

Figure Lengend Snippet: A) Each column shows images of the same field, with the fluorescence of the green fluorescent protein (GFP) in the top row and the differential interference contrast (DIC) images of the cultured yeast cells in the bottom row. The Δ stf2 cells transformed with the vector expressing GFP-STF2 under the control of the GAL promoter were photographed after 4 h of galactose or glucose supplementation. Cells expressing the GFP-STF2 fusion protein under the STF2 promoter were photographed after 24 h in the stationary phase. B) Analysis of cells expressing GFP-STF2p using the confocal microscope. Image generated by the average of a pile of five optical sections. C) The scale of viability (%) indicates the percentage of the experimental values for the different strains after the dehydration and rehydration process relative to the highest value for the fresh cultures before the induction of the stress. Values are the means of n = 3 determinations ± the SD. *Significant differences ( p ≤0.01) of overexpressing strains with respect to the BY4742, GALpG strain.

Article Snippet: Confocal images were obtained using a laser microscope (Nikon TE2000-E) equipped with a digital camera (Nikon DXM1200C), and overlaid images using NIS-Elements software (Nikon).

Techniques: Fluorescence, Cell Culture, Transformation Assay, Plasmid Preparation, Expressing, Control, Microscopy, Generated